Endocrine Abstracts

Introduction: Multinodular Goiter (MNG) is a thyroid neoplastic disease thirteen times more common in women than in men. While a local effect of estrogens at cellular levels could affect there can be other causes to explain the gender difference in incidence such as diet, environmental factors, genetic causes or alterations in metabolic pathways.Cellular effects of estrogens in human thyrocytes although previously studied are not completely clarified. So...

Up to now there are few studies about differential proteomics in thyroid. Our group has developed a method for standard human primary thyroid culture maintaining the phenotype through passages. Thus, we believe of great interest the search for biomarkers in these cultures by 2D-PAGE coupled to MALDI-TOF/TOF-MS.Aims: i) To standardize the conditions for sample extraction and controls. ii) To compare and identify the differential proteomic pattern between ...

Toxic adenomas (TA) and toxic multinodular goiters (TMNG) are frequent causes of hyperthyroidism in Galicia, an endemic goiter area. In some European countries, 40–80% of toxic goiters are caused by TSH receptor (TSHr) and Gs alfa-subunit (Gsa) mutations that activate cAMP pathway.Aims: To study 1) the prevalence of TSHr and Gsa mutations in TMNG and TA from Galicia, 2) the clonality of sequenced samples, 3) the constitutive activity of the identifi...